Improve Research Reproducibility A Bio-protocol resource
- Protocols
- Articles and Issues
- For Authors
- About
- Become a Reviewer
Categories
Antimicrobial assay Community analysis Heterologous expression system in vivo model Microbe-host interactions Microbial biochemistry Microbial biofilm Microbial cell biology Microbial genetics Microbial metabolism
Microbial physiology Adaptation
Cell wall
Chemotaxis
Competence
Interspecific competition
Ion transport
Membrane property
Photosynthesis
Phototaxis
Respiration
Stress response
Microbial proteomics Microbial signaling Pathogen detectionDetermination of the in vitro Sporulation Frequency of Clostridium difficile
The anaerobic, gastrointestinal pathogen, Clostridium difficile, persists within the environment and spreads from host-to-host via its infectious form, the spore. To effectively study spore formation, the physical differentiation of vegetative cells from spores is required to determine the proportion of spores within a population of C. difficile. This protocol describes a method to accurately enumerate both viable vegetative cells and spores separately and subsequently calculate a sporulation frequency of a mixed C. difficile population from various in vitro growth conditions (Edwards et al., 2016b).